Recent publications by Helsinki Eye Lab are listed below.
  • Viitaja, T.; Moilanen, J.; Raitanen, J.-E.; Hynynen, A.; Moilanen, J.; Svedström, K.; Paananen, R. O.; Ekholm, F. S. On the Importance of Chain Branching in Tear Film Lipid Layer Wax and Cholesteryl Esters. Colloids and Surfaces B: Biointerfaces XX, XXXX–XXXX (2022). DOI:
  • Viitaja, T.; Moilanen, J.; Svedström, K. J.; Ekholm F. S.; Paananen, R. O. Tear Film Lipid Layer Structure: Self-Assembly of O-Acyl-ω-hydroxy Fatty Acids and Wax Esters into Evaporation-Resistant Monolayers. Nano Letters 21, 7676–7683 (2021). DOI:
  • Viitaja, T.; Raitanen, J.-E.; Moilanen, J.; Paananen, R. O; Ekholm, F. S. The Properties and Role of O-Acyl-ω-hydroxy Fatty Acids and Type I-St and Type II Diesters in the Tear Film Lipid Layer Revealed by a Combined Chemistry and Biophysics Approach. Journal of Organic Chemistry 86, 4965-4976 (2021). DOI:
  • Paananen, R. O.; Viitaja, T.; Olżyńska, A.; Ekholm, F. S.; Moilanen, J.; Cwiklik, L. Interactions of Polar Lipids with Cholesteryl Ester Multilayers Elucidate Tear Film Lipid Layer Structure. The Ocular Surface 18, 545-553 (2020). DOI:
  • Liekkinen, J.; de Santos Moreno, B.; Paananen, R. O.; Vattulainen, I.; Monticelli, L.; de la Serna, J. B.; Javanainen, M. Understanding the Functional Properties of Lipid Heterogeneity in Pulmonary Surfactant Monolayers at the Atomistic Level. Frontiers in Cell and Developmental Biology 8, 581016 (2020). DOI:
  • Paananen, R. O.; Javanainen, M.; Holopainen, J. M.; Vattulainen, I. Crystalline Wax Esters Regulate the Evaporation Resistance of Tear Film Lipid Layers Associated with Dry Eye Syndrome. Journal of Physical Chemistry Letters 10, 3893-3898 (2019). DOI:
  • Bland, H.; Moilanen, J.; Ekholm, F. S.; Paananen, R. O. Investigating the Role of Specific Tear Film Lipids Connected to Dry Eye Syndrome: A Study on O-acyl-ω-hydroxy-fatty acids and Diesters. Langmuir 35, 3545-3552 (2019). DOI:
  • Ruokonen, S-K.; Sanwald, C.; Robciuc, A.; Hietala, S.; Rantamäki, A. H.; Witos, J.; King, A. W. T.; Lämmerhofer, M.; Wiedmer, S. K. Correlation between Ionic Liquid Cytotoxicity and Liposome-Ionic Liquid Interactions. Chemistry: A European Journal 24, 2669-2680 (2018). DOI:
  • Robciuc, A.; Arvola, R. P. J.; Jauhiainen, M.; Holopainen, J. M. Matrix regeneration agents improve wound healing in non-stressed human corneal epithelial cells. Eye 32, 813-819 (2018). DOI:
  • Sahraravand, A.; Haavisto, A.-K.; Holopainen, J. M.; Leivo, T. Ocular trauma in the Finnish elderly – Helsinki Ocular Trauma Study. Acta Ophthalmologica 96, 616-622 (2018). DOI:
  • Garrigue, J.-S.; Amrane, M.; Faure, M.-O.; Holopainen, J.M.; Tong L. Relevance of Lipid-Based Products in the Management of Dry Eye Disease. Journal of Ocular Pharmacology and Therapeutics 33, 647-661 (2017). DOI:
  • Haavisto, A-K.; Sahraravand, A.; Holopainen, J.M.; Leivo, T. Paediatric eye injuries in Finland – Helsinki eye trauma study. Acta Ophthalmologica 95, 392-399 (2017). DOI:
  • Nelson, J.D.; Craig, J.P.; Akpek, E.K.; Azar, D.T.; Belmonte, C.; Bron, A.J.; Clayton, J.A.; Dogru, M.; Dua, H.S.; Foulks, G.N.; Gomes, J.A.P.; Hammitt, K.M.; Holopainen, J.M.; Jones, L.; Joo, C-K.; Liu, Z.; Nichols, J.J.; Nichols, K.K.; Novack, G.D.; Sangwan, V.; Stapleton, F.; Tomlinson, A.; Tsubota, K.; Willcox, M.D.P.; Wolffsohn, J.S.; Sullivan, D.A. TFOS DEWS II Introduction. Ocular Surface 15, 269-275 (2017). DOI:
  • Willcox, M.D.P.; Argueso, P.; Georgiev, G.A.; Holopainen, J.M.; Laurie, G.W.; Millar, T.J.; Papas, E.B.; Rolland, J.P.; Schmidt, T.A.; Stahl, U.; Suarez, T.; Subbaraman, L.N.; Ucakhan, O.O.; Jones, L. TFOS DEWS II Tear Film Report. Ocular Surface 15, 366-403 (2017). DOI:
  • Robciuc, A.; Witos, J.M.; Ruokonen, S-K.; Rantamäki, A.H.; Pisella, P-J.; Wiedmer, S.K.; Holopainen, J.M. Pure Glaucoma Drugs Are Toxic to Immortalized Human Corneal Epithelial Cells, but They Do Not Destabilize Lipid Membranes. Cornea 36, 1249–1255 (2017). DOI:
  • Sahraravand, A.; Haavisto, A.; Holopainen, J.M.; Leivo, T. Ocular traumas in working age adults in Finland - Helsinki Ocular Trauma Study. Acta Ophthalmologica 95, 288-294 (2017). DOI:
  • Rantamäki, A.H.; Holopainen, J.M. The Effect of Phospholipids on Tear Film Lipid Layer Surface Activity. Investigative Ophthalmology & Visual Science 58, 149-154 (2017). DOI:
  • Ruokonen, S.; Dusa F.; Rantamäki, A.H.; Robciuc, A.; Holma, P.; Holopainen, J.M.; Abdel-Rehim, M.; Wiedmer, S.K. Distribution of local anesthetics between aqueous and liposome phases. Journal of Chromatography. A 1479, 194-203 (2017). DOI:


  • Mattila, J.S.; Holopainen, J.M. Bilateral Ectasia After Femtosecond Laser-Assisted Small Incision Lenticule Extraction (SMILE). Journal of Refractive Surgery 32, 497-500 (2016). DOI:
  • Arvola, R.P.J.; Robciuc, A.; Holopainen, J.M. Matrix Regeneration Therapy: A Case Series of Corneal Neurotrophic Ulcers. Cornea 35, 451-455 (2016). DOI:
  • Kivinen, N.; Felszeghy, S.; Kinnunen, A.I.; Setälä, N.; Aikio, M.; Kinnunen, K.; Sironen, R.; Pihlajaniemi, T.; Kauppinen, A.; Kaarniranta, K. Absence of collagen XVIII in mice causes age-related insufficiency in retinal pigment epithelium proteostasis. Biogerontology 17, 749-761 (2016). DOI:
  • Koisaari, T.; Michelsson, K.; Holopainen, J.M.; Maksimainen, R.; Paivansalo, J.; Rantala, K.; Tervo, T. Traffic and Criminal Behavior of Adults with Attention Deficit-Hyperactivity with a Prospective Follow-Up from Birth to the Age of 40 Years. Traffic injury prevention 16, 824-830 (2015). DOI:
  • Serra, H.M.; Holopainen, J.M.; Beuerman, R.; Kaarniranta, K.; Suarez, M.F.; Urrets-Zavalia, J.A. Climatic droplet keratopathy: an old disease in new clothes. Acta Ophthalmologica 93, 496-504 (2015). DOI:
  • Wetterstrand, O.; Holopainen, J.M.; Krootila, K. Femtosecond Laser-Assisted Intrastromal Relaxing Incisions After Penetrating Keratoplasty: Effect of Incision Depth. Journal of Refractive Surgery 31, 474-479 (2015). DOI:
  • Mattila, J.S.; Krootila, K.; Kivelä, T.; Holopainen, J.M. Penetrating Keratoplasty for Corneal Amyloidosis in Familial Amyloidosis, Finnish Type. Ophthalmology 122, 457-463 (2015). DOI:
  • Mikkola, S.; Robciuc, A. Lokajova, J.; Holding, A.J.; Laemmerhofer, M.; Kilpelainen, I.; Holopainen, J.M.; King, A.W.T.; Wiedmer, S.K. Impact of Amphiphilic Biomass-Dissolving Ionic Liquids on Biological Cells and Liposomes. Environmental science & technology 49, 1870-1878 (2015). DOI:
  • Paananen, R.O.; Rantamäki, A.H.; Parshintsev, J.; Holopainen, J.M. The Effect of Ambient Ozone on Unsaturated Tear Film Wax Esters. Investigative Ophthalmology & Visual Science 56, 8054-8062 (2015). DOI:
  • Hautamäki, A.; Seitsonen, S.; Holopainen, J.M.; Moilanen, J.A.; Kivioja, J.; Onkamo, P.; Järvelä, I.; Immonen, I. The genetic variant rs4073 A→T of the Interleukin-8 promoter region is associated with the earlier onset of exudative age-related macular degeneration. Acta Ophthalmologica 93, 726-733 (2015). DOI:
  • Järventausta, P.J.; Tervo, T.M.T.; Kivelä, T.; Holopainen, J.M. Peripheral hypertrophic subepithelial corneal degeneration - clinical and histopathological features. Acta Ophthalmologica 92, 774-782 (2014). DOI:
  • Kulovesi, P.; Rantamäki, A.H.; Holopainen, J.M. Surface Properties of Artificial Tear Film Lipid Layers: Effects of Wax Esters. Investigative Ophthalmology & Visual Science 55, 4448-4454 (2014). DOI:
  • Loukovaara, S.; Lehti, K.; Robciuc, A.; Pessi, T.; Holopainen, J.M.; Koli, K.; Immonen, I.; Keski-Oja, J. Increased intravitreal angiopoietin-2 levels associated with rhegmatogenous retinal detachment. Graefe's Archive for Clinical and Experimental Ophthalmology 252, 881-888 (2014). DOI:
  • Paananen, R.O.; Rantamaki, A.; Holopainen, J.M. Antievaporative Mechanism of Wax Esters: Implications for the Function of Tear Fluid. Langmuir 30, 5897-5902 (2014). DOI:
  • Robciuc, A.; Rantamäki, A.H.; Jauhiainen, M.; Holopainen, J.M. Lipid-Modifying Enzymes in Human Tear Fluid and Corneal Epithelial Stress Response. Investigative Ophthalmology & Visual Science 55, 16-24 (2014). DOI:
  • Franova, M.D.; Repakova, J.; Holopainen, J.M.; Vattulainen, I. How to link pyrene to its host lipid to minimize the extent of membrane perturbations and to optimize pyrene dimer formation. Chemistry and Physics of Lipids 177, 19-25 (2014). DOI:
  • Jarventausta, P.J.; Holopainen, J.M.; Zalentein, W.N.; Paakkanen, R.; Wennerstrom, A.; Seppanen, M.; Lokki, M-L.; Tervo, T.M.T. Peripheral hypertrophic subepithelial corneal degeneration: characterization, treatment and association with human leucocyte antigen genes. Acta Ophthalmologica 92, 71-76 (2014). DOI:
  • Rantamäki, A.H.; Wiedmer, S.K.; Holopainen, J.M. Melting Points-The Key to the Anti-Evaporative Effect of the Tear Film Wax Esters. Investigative Ophthalmology & Visual Science 54, 5211-5217 (2013). DOI:
  • Robciuc, A.; Hyotylainen, T.; Jauhiainen, M.; Holopainen, J.M. Ceramides in the Pathophysiology of the Anterior Segment of the Eye. Current Eye Research 38, 1006-1016 (2013). DOI:
  • Loukovaara, S.; Robciuc, A.; Holopainen, J.M.; Lehti, K.; Pessi, T.; Liinamaa, J.; Kukkonen, K-T.; Jauhiainen, M.; Koli, K.; Keski-Oja, J.; Immonen, I. Ang-2 upregulation correlates with increased levels of MMP-9, VEGF, EPO and TGF beta 1 in diabetic eyes undergoing vitrectomy. Acta Ophthalmologica 91, 531-539 (2013). DOI:
  • Wetterstrand, O.; Holopainen, J.M.; Krootila, K. Treatment of Postoperative Keratoplasty Astigmatism Using Femtosecond Laser-Assisted Intrastromal Relaxing Incisions. Journal of Refractive Surgery 29, 378-382 (2013). DOI:
  • Tervo, T.M.T.; Räty, E.; Sulander, P.; Holopainen, J.M.; Jaakkola, T.I.; Parkkari, K. Sudden death at the wheel due to a disease attack. Traffic injury prevention 14, 138-144 (2013). DOI:
  • Telenius, J.; Koivuniemi, A.; Kulovesi, P.; Holopainen, J.M.; Vattulainen, I. Role of Neutral Lipids in Tear Fluid Lipid Layer: Coarse-Grained Simulation Study. Langmuir 28, 17092-17100 (2012). DOI:
  • Lokajova, J.; Holopainen, J.M.; Wiedmer, S.K. Comparison of lipid sinks in sequestering common intoxicating drugs. Journal of Separation Science 35, 3106-3112 (2012). DOI:
  • Vuori, E.; Vanni, S.; Henriksson, L.; Tervo, T.M.T.; Holopainen, J.M. Refractive surgery in anisometropic adult patients induce plastic changes in primary visual cortex. Acta Ophthalmologica 90, 669-676 (2012). DOI:
  • Robciuc, A.; Hyötyläinen, T.; Jauhiainen, M.; Holopainen, J.M. Hyperosmolarity induced lipid droplet formation depends on ceramide production by neutral sphingomyelinase 2. Journal of Lipid Research 53, 2286-2295 (2012). DOI:
  • Litonius, E.; Lokajova, J.; Yohannes, G.; Neuvonen, P.J.; Holopainen, J.M.; Rosenberg, P.; Wiedmer, S. In vitro and in vivo entrapment of bupivacaine by lipid dispersions. Journal of Chromatography A 1254, 125-131 (2012). DOI: 10.1016/j.chroma.2012.07.013
  • Wiedmer, S.K.; Robciuc, A.; Kronholm, J.; Holopainen, J.M.; Hyötyläinen, T. Chromatographic lipid profiling of stress-exposed cells. Journal of Separation Science 35, 1845-1853 (2012). DOI:
  • Rantamäki, A.; Javanainen, M.; Vattulainen, I.; Holopainen, J.M. Do Lipids Retard the Evaporation of the Tear Fluid? Investigative Ophthalmology & Visual Science. 53, 6442-6447 (2012). DOI:
  • Holopainen, J.M.; Robciuc, A.; Cafaro, T.A.; Suarez, M.F.; Konttinen, Y.; Alkatan, H.M.; Tabbara, K.F.; Tervahartiala, T.; Sorsa, T.; Urrets-Zavalia, J.A.; Serra, H.M. Pro-Inflammatory Cytokines and Gelatinases in Climatic Droplet Keratopathy. Investigative Ophthalmology & Visual Science. 53, 3527-3535 (2012). DOI:
  • Kulovesi, P.; Telenius, J.; Koivuniemi, A.; Brezesinski, G.; Vattulainen, I.; Holopainen, J.M. The impact of lipid composition on the stability of the tear fluid lipid layer. Soft Matter. 8, 5826-5834 (2012). DOI: