ViPS Invited Seminar June 2018
Date: 6th June 2018
Title: Signals and receptors involved in endosymbiosis
Location: Biocentre 3, seminar room 2402, Viikinkaari 1
Host: Alan Schulman
Abstract: My research activities have for most of my career had a strong focus and legumes and their interaction with microorganisms. Legumes form endosymbioses with rhizobia and arbuscular mycorrhizal fungi, host endophytes, support a rhizosphere community and like other plants they are attacked by pathogens. One of the features enabling legumes to distinguish between these very different microbes appears to be a large family of LysM receptor kinases monitoring microbial signals. LysM receptor kinases have been shown to play a crucial role for perception of rhizobial Nod factors while others have not been studied. The function of some of these receptors in perception of signal molecules including lipochito-oligosaccharides, exopolysaccharides and chitin derived signal molecules and in plant-microbe interaction will be presented together with the genetic and biochemical methods used for functional studies. Biochemical approaches for detailed characterization of ligand – LysM receptor interactions will be presented and a model for legume recognition of rhizobial bacteria and pathogens will be discussed.
Jens Stougaard is Professor of Molecular Biology and Genetics at Aarhus University and Director of the Centre for Carbohydrate Recognition and Signalling (CARB). Jens Stougaard leads a group studying plant genes regulating the development of nitrogen fixing root nodules and mycorrhiza formation in legumes. Currently the perception mechanisms for the bacterial Nod-factor and exopolysaccharide signal molecules, the function of receptors involved and the downstream signal transduction cascades are in focus. The plant model system used for this research is Lotus japonicus that form determinate root nodules. Lotus japonicus is also used for investigating the long-range signalling integrating root nodule development into the general developmental program of the plant. Genetics, genomics and biochemical methods are used to identify and characterise components of regulatory circuits including hormone regulation of nodule organogenesis and rhizobial infection. In order to improve the genetic analysis, a large-scale insertion population based on the germ-line specific activity of the LORE1 retroelement has been established and made available to the community as a resource for reverse genetics in plants.
More recently a more strategic research project aiming to develop genomics-based breeding of Vicia faba (faba bean) has been initiated with collaborators from Denmark, UK, Finland and Canada. The idea is to build a consortium of Northern hemisphere players, NORFAB (NORthern FABa) with the critical mass and key expertise required to develop sophisticated genomics-based plant breeding methods and provide access to germplasm with the relevant genetic diversity. NORFAB aims to improve the yield and quality of faba bean seeds to a level making faba bean a competitive protein crop for cultivation on arable farmland under northern European and Canadian/US northern prairie conditions. NORFAB will establish genomics and genetics and use new genotyping and phenotyping methodologies to develop this orphan crop.