Leica Stellaris 8 DIVE Falcon

(Room AP29b)

Upright multiphoton & confocal microscope optimized for intravital imaging of anesthetized small laboratory animals.

The system allows acute as well chronic in vivo imaging experiments on internal (e.g. brain, kidney, liver etc.) and external organs (e.g. skin and eye). The system can also be used for imaging of thick tissue samples/explants.

Detectors:

  • 2 non-descanned hybrid detectors & 1 non-descanned PMT detector for multiphoton imaging
  • 3 internal hybrid detectors for confocal imaging
  • adjustable filtering

Objectives:

  • 25x water (HC IRAPO L 25x/1.00 W motCORR)

Excitation lasers:

Coherent Chameleon Discovery NX

  • tunable line 660-1320 nm
  • fixed line 1040 nm

3 single photon (confocal) lasers

  • 488 nm
  • 561 nm
  • 638 nm

Accessories:

  • Leica FALCON FLIM module
  • Dark microscope enclosure
  • Univentor gas anesthesia system with mask, induction boxes and scavenger
  • PhysioSuite heart/breath rate and oxygenation monitoring system for mice and rats
  • Animal heating pad at the microscope stage to keep the animal warm under anesthesia
  • Custom-made adjustable motorized microscope stage with a variety of intravital animal fixation adapters
  • LED-light source F1 with flexible lightguides for manipulations under the microscope
  • Motorized microscope stage