In Finland the species is exclusively associated with the pedunculate oak (Quercus robur), within other parts of its distributional range, Tischeria ekebladella also feeds on other oak species and chestnut. In Finland the species has one generation per year. The adult moths fly in June-July, eggs are laid on the upper side of the leaves. The larvae form white mines, irregular in shape, under the upper epidermis of the leaf. The larvae overwinter in circular-shaped cocoons within the abscised leaves. Tischeria ekebladella is both common and abundant in south-western Finland.