Suitable for patient research, clinical cohorts and research projects.
Required sample amount: >10000 cells, 1.5 mg tissue/solid or 10 µl fluid.
Sample types: biofluids (serum, plasma, blood, CSF, sweat, tears), tissues (brain, muscle, caecum, gut, skin and mucus) and others (feces, cells, organoids, spheres, bacteria, extracellular vesicles).
Throughput approximately 150 samples per week.
Identification and within run relative quantitation of more than 400 metabolites using high resolution orbitrap mass spectrometer. The exact number of identified metabolites is dependent on sample matrix and sample amount.
New matrix types are welcome but require pilot testing.
Results are provided in excel format ready for further data analysis.
Collaboration article DOI:10.1126/sciadv.adf7119, check publication list for others.
Suitable for patient research, clinical cohorts and research project. Suitable also for sample amount 1-500, sample sizes 1milj cells, 100ul. Analysis of hundreds of polar metabolites in a single (LC-MS Waters MRM. suitable for tissues, cells, and biofluids (https://doi.org/10.3390/metabo8030044, check publication list for others). Data provided in excel format ready for data analysis.
Suitable for patient research, clinical cohorts and research projects. Required sample amount: 1-6x10e6 cells or 50 µl blood. Throughput approximately 150 samples per week. Identification and absolute quantitation with internal standards using high resolution orbitrap mass spectrometer. New matrix types are welcome but require pilot testing. Results are provided in excel format ready for further data analysis.
Analytes: propionic acid, acetic acid, butyric/isobutyric acid, valeric acid, isovaleric acid, 2-methylvaleric acid, 3-methylvaleric acid. Suitable for patient research, clinical cohorts and research projects. Throughput approximately 150 samples per week. Sample types: biofluids (serum, plasma, blood), tissues, others (feces, cells, organoids, spheres, bacteria, extracellular vesicles). Identification and absolute quantitation with internal standards using high resolution orbitrap mass spectrometer. New matrix types are welcome but require pilot testing. Results are provided in excel format ready for further data analysis.
Analytes: dopamine and norepinephrine. Suitable for research projects. Throughput approximately 50 samples per week. Sample types: CSF and cells. Identification and absolute quantitation with internal standards using MRM method on Qtrap mass spectrometer. New matrix types are welcome but require pilot testing. Results are provided in excel format ready for further data analysis.
Quantitative LC-MS concentration measurement using iSTD with MRM or high-resolution orbitrap Q-Exactive. Concentration analysis 1) folate cycle intermediates (check publication list) 2) ketonebodies 2) ketoacids, 3) absolute quantification of TCA cycle intermediates (https://doi.org/10.3390/metabo10030103). Suitable for tissues, cells, and biofluids. Requires matrix effect validation if a new sample type.
Isotope enrichment analysis and metabolic flux analysis of central carbon metabolism, glucose, NADolome, glutamine, other pathways using 13C-, 15N- and dH-tracers. Suitable also for small sample amounts (i.e. 30 000 cells or 10ul) for biofluids, tissues, cells, immune cells, stem cells, organoids, spheres, and extracellular vesicles. Collaboration article https://doi.org/10.1016/j.redox.2022.102416, check publication list for others. Requires consultation and collaboration.
Analysis of thousand lipid molecular species in a single method using the Lipidyzer platform. Suitable for biofluids (min 25ul), tissues, cells, and extracellular vesicles. More details doi: 10.1021/acs.analchem.1c02826
We offer via collaboration help for metabolomics/lipidomics data analysis. Contact the head of the unit.
We are open to develop via collaboration new metabolomics analytical methods both for precision metabolomics and research purposes. Contact the head of the unit.
We are open for consulting patient and clinical metabolomics work, teaching, seminar and conference talks. Contact the head of the unit.