Leica SP8 CARS

Leica TCS SP8 MP CARS confocal

(Room B116a)

 

Laser scanning confocal microscope for both fixed and live cell imaging. Mounted on a motorized Leica inverted microscope.

Detectors:

  • 2 high sensitivity hybrid detectors (HyD)
  • 1 PMT detector
  • 2 reflected light non-descanned PMT detectors (with possibility for filters)
  • 2 transmitted light non-descanned PMT detectors (with possibility for filters)

Objectives:

  1. 10x multi-immersion
  2. 25x water
  3. 20 multi-immersion
  4. 40x water
  5. 63x water
  6. 63x glycerol

Single photon laser lines:

  • Diode 405 nm
  • Argon
    • 458 nm
    • 476 nm
    • 488 nm
    • 496 nm
    • 514 nm
  • DPSS 561 nm
  • He-Ne 594 nm
  • He-Ne 633 nm

Multi photon laser

  • APE picoEmerald CARS picosecond multiphoton laser

Environmental Chamber operating temperature

The LSM 880 has a full environmental chamber for imaging live cells. While the chamber and microscope are heating up or cooling down the light path distances are constantly changing. Because of this it is not recommended to image while the chamber temperature is changing.

To keep the chamber at a steady temperature, it should be either heated to (+37 degrees Celsius) or at room temperature for the whole day.

The first reservation created for a given day determines the temperature of the system.

Mark the desired temperature in the "Description" field of the reservation on Scheduler. Do this even if you're not the first user to reserve the microscope. The first user of the day should turn on the heating if the system is reserved for +37.

The heating is equipped with a timer, so it can be set to turn on before the start of your reservation. It will take at least 1 h for the heating to stabilize the temperature and at least two hours for it to cool down to room temperature.

NB! If you are imaging fixed cells, you can do the imaging at +37 too.