Helsinki Virus (HelVi) Cores

Biomedicum Virus Core-BVC

AAV Gene Transfer and Cell Therapy Core Facility

The Helsinki Virus (HelVi) Core is a service platform that provides generation of recombinant adeno-associated viral (rAAV) preps for academic and industrial customers in Finland as well as abroad. HelVi uses biosafety level I laboratory equipped with all necessary cell culture equipment such as laminar hoods, incubators, centrifuges and microscopes.

Contact persons: Andrey Anisimov, +3582941 25562, andrey.anisimov@helsinki.fiTanja Laakkonen, +3582841 25563, tanja.laakkonen@helsinki.fiKari Alitalo, Core director, +3582941 25511,

Location: Biomedicum Helsinki, 5th floor, Haartmaninkatu 8, Meilahti Campus

Brief introduction to the technology - 5 easy steps

  1. The gene-of-interest or shRNA are cloned into the AAV plasmid vector
  2. Generation of rAAV particles carrying the gene of interest or shRNA
  3. Administration of rAAV particles into cells or experimental animals
  4. rAAV transduces host cells and releases its recombinant genome
  5. The transgenic protein or shRNA is expressed


  • Manufacturing rAAV preps from the customer’s gene-encoding plasmids. The available capacity allows the production of 3xE+11 to 3xE+12 virus genomes/ml in one purification round. The currently used serotypes are 2, 6, 8 and 9
  • Routine functional tests of the rAAV preps to characterize transduction efficiency and the ability to produce the transgenic protein in transduced host cells
  • Providing customers with control rAAV preps, immediately available upon request
  • Consulting the customers regarding rAAV vector design, tissue/cell specificity of transduction, fluorescent markers, tags, methods to increase expression level and bioavailability
  • Researcher training tailored to client needs
  • Generation of CRISPR rAAV preps for genome editing


  • Heracell 150 CO2 cell culture incubator (2 x)
  • KOJAIR cell culture hood for the viral work
  • Sorvall RC 6+ centrifuge equipped with rotors
  • A&D EK-400H scale
  • Ultracentrifuge Optima XL-80K Beckman Coulter
  • EVOS ® FL Imaging System
  • Olympus CK2 inverted bright field microscope
  • Zeiss AxioImager and Zeiss LSM780 and 880 microscopes


  • Recombinant virus production and quantification
  • Quality test for the ready viral preps
  • Optimization of AAV vectors upon request

User fees

The research infrastructure is supported by University of Helsinki (HiLIFE and Research Programs Unit) and Biocenter Finland. The prices for University of Helsinki customers (VAT 0 %) are listed below. For the other customers, the VAT is 24%.

Service Fee
Internal customers 676,86 €
HUS customers 676,86 €
External academic customers 1050,27 €
External customers 1397,43 €

Ready AAV preps available upon request

HelVi has several ready AAV preps in storage, which can be mainly used as a negative control in vivo or in vitro. These ready AAV preps * (as listed in the Table below) can be sold in smaller amounts, reducing the price.

Transgene AAV serotype Notes
Human Serum Albumin (HSA) AAV9  
Empty vector (no transgene) AAV9  
Fc-part of mouse IgG (mFC) AAV9, AAV2 Control for Fc-fuced proteins
Luciferaasi AAV9, AAV2, AAV8  
LacZ AAV9  
Cre recombinase AAV9, AAV2, AAV8 Can be used to cut away loxP flanked DNA sequences

* For other available AAV preps, not included in the Table, please, send e-mail or call.


The Biomedicum Virus Core (BVC)

BVC is a service platform that provides biosafety level II laboratory space for recombinant virus production as well as consultation, technical help and gene transfer products for the researchers on the Meilahti and Viikki Campuses.

BVC is equipped with basic cell culture equipment such as laminars, incubators, centrifuges and microscopes. Latest purchase for BVC is a new fluorescent microscope. All registered users are allowed to use the EVOS® FL Imaging System. The microscope will be reserved through the BVC Online Hood Reservation Calendar. (

For further information, please visit

Contact person: Katariina Karjalainen (Tiina Raatikainen on maternity leave), BVC Management, +3582941 25494,,

Location: Biomedicum Helsinki, 5th floor, Haartmaninkatu 8, Meilahti Campus


  • Registration & Biosafety Training
  • General facilities supervision and support
  • Hood and facility use
  • Biosafety test (RCV test)
  • Virus titering test for lentiviral particles (p24 test)
  • General cell lines related to virus work
  • Transfection reagent aliquotes


  • Thermo Forma Series II Water Jacketed CO2 Incubator (2 x)
  • Heraeus Multifuge 3S-R (w/ tube and plate rotor)
  • Olympus CK40 bright field microscope
  • A&D EK-400H scale
  • Labnet International Spectrafuge 24 D
  • Wealtec E-centrifuge
  • Hettich Universal 32 centrifuge
  • Memmert waterbath
  • Sartorius Biohit MidiPlus pipettors
  • Stuart vortex
  • EVOS® FL Imaging System


  • Recombinant virus production
  • Transduction of target cells
  • Fluorescent wide-field imaging for BSL2 substances

User fees

The research infrastructure is supported by University of Helsinki (HiLIFE and Research Programs Unit) and Biocenter Finland. The prices for University of Helsinki customers (VAT 0 %) are listed below. Other customers, please contact

Service Price (eur)

Registration & Biosafety training (€/person)


Usage of the facility (1h)

RCV test 45
p24 test 55
Cell ampoul 10
Transfection reagent 200