Instruments
At this moment, Biocenter Flow Cytometry unit has two flow cytometers, BD LSR II and BD LSRFortessa. The instruments are located at Biocenter 1 in Viikki Campus, Viikinkaari 9 C, 4th floor, room 4402.
For flow cytometry analysis with cell sorting or when needing a laser configuration we cannot provide, we kindly ask you to contact:
LSR II
The BD LSR II Flow Cytometer analyser can be used after training. BD LSR II has two lasers:
- blue 488 nm
- red 633 nm
You can analyze up to 6 fluorescence and 2 scatter parameters simultaneously. Samples can be analyzed with 3 different flow rates: low (12 µL/min), medium (35 µL/min) and high (60 µL/min). Flow rate can also be adjusted with sample fine adjustment (6-24, 17.5-70 and 30-120 µL/min, respectively). LSR II uses BD FACSDiva Software v 8.0.
LSRFortessa
The BD LSRFortessa Flow Cytometer analyser can be used after training. BD LSRFortessa is equipped with HTS sample loader and contains 4 lasers:
- violet 405 nm
- blue 488 nm
- yellow green 561 nm
- red 640 nm
You can analyze up to 16 fluorescence and 2 scatter parameters simultaneously. Samples can be analyzed with 3 different flow rates: low (12 µL/min), medium (35 µL/min) and high (60 µL/min). Flow rate can also be adjusted with sample fine adjustment (6-24, 17.5-70 and 30-120 µL/min, respectively). BD LSRFortessa uses BD FACSDiva Software v 8.0.
HTS Sample Loader
The HTS option is compatible with 96-well U, V, and flat-bottom plates as well as 384-well microtiter plates. Customers are asked to use recommended plates as otherwise the plate dimensions can cause problems.
Compatible BD Falcon Microtiter Plates:
- 96-well U bottom (Cat. No. 353910)
- 96-well V bottom (Cat. No. 353263)
- 96-well flat bottom (Cat. No. 353915)
- 384-well flat bottom (Cat. No. 353233)
Our instruments run on FACSDiva 8.0 and we offer our customers different softwares for data analysis