Instruments

The BD LSR II Flow Cytometer analyser can be used after training. BD LSR II has two lasers:

  • blue 488 nm
  • red 633 nm

You can analyze up to 6 fluorescence and 2 scatter parameters simultaneously. Samples can be analyzed with 3 different flow rates: low (12 µL/min), medium (35 µL/min) and high (60 µL/min). Flow rate can also be adjusted with sample fine adjustment (6-24, 17.5-70 and 30-120 µL/min, respectively). LSR II uses BD FACSDiva Software v 8.0.

Detectors

Optical Filters

BD LSR II User's Guide

 

The BD LSRFortessa Flow Cytometer analyser can be used after training. BD LSRFortessa is equipped with HTS sample loader and contains 4 lasers:

  • violet 405 nm
  • blue 488 nm
  • yellow green 561 nm
  • red 640 nm

You can analyze up to 16 fluorescence and 2 scatter parameters simultaneously. Samples can be analyzed with 3 different flow rates: low (12 µL/min), medium (35 µL/min) and high (60 µL/min). Flow rate can also be adjusted with sample fine adjustment (6-24, 17.5-70 and 30-120 µL/min, respectively). BD LSRFortessa uses BD FACSDiva Software v 8.0.

Detectors

Optical Filters

BD LSRFortessa User's Guide

 

 

The HTS option is compatible with 96-well U, V, and flat-bottom plates as well as 384-well microtiter plates. Customers are asked to use recommended plates as otherwise the plate dimensions can cause problems.

Compatible BD Falcon Microtiter Plates:

  • 96-well U bottom (Cat. No. 353910)
  • 96-well V bottom (Cat. No. 353263)
  • 96-well flat bottom (Cat. No. 353915)
  • 384-well flat bottom (Cat. No. 353233)

HTS Quick Reference Guide

HTS User's Guide

HTS Sample Loader