LOCUS       p1R4_ML_XVE             5983 bp ds-DNA     circular     10-DEC-2015
DEFINITION  Complementary copy of Entry Clone/pGEM-TP4P1R/MLXVE.
SOURCE      
  ORGANISM  
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             60..1511
                     /vntifkey="4"
                     /label=XVE
                     /ApEinfo_fwdcolor=#ff0080
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     3'UTR           1531..1625
                     /vntifkey="50"
                     /label=3'UTR
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     terminator      1651..1945
                     /vntifkey="43"
                     /label=rbcST
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     terminator      1954..2229
                     /vntifkey="43"
                     /label=nosT
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2240..2510
                     /vntifkey="21"
                     /label=LexA
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        2532..2590
                     /vntifkey="29"
                     /label=minim\35S
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(4109..4969)
                     /vntifkey="21"
                     /label=AmpR
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2601..2724
                     /vntifkey="21"
                     /label=attR1
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1..8
                     /vntifkey="21"
                     /label=attL4
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     5874..5893
                     /label=1R4_F Seq primer
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(189..208)
                     /label=XVE_3R Seq primer
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 aaaagttggc gcgccacgat accggtggta ccctcgagta cgtaccaatt ccgggcggaa
       61 tgaaagcgtt aacggccagg caacaagagg tgtttgatct catccgtgat cacatcagcc
      121 agacaggtat gccgccgacg cgtgcggaaa tcgcgcagcg tttggggttc cgttccccaa
      181 acgcggctga agaacatctg aaggcgctgg cacgcaaagg cgttattgaa attgtttccg
      241 gcgcatcacg cgggattcgt ctgttgcagg aagaggaaga agggttgccg ctggtaggtc
      301 gtgtggctgc cggtgaaccg tcgagcgccc ccccgaccga tgtcagcctg ggggacgagc
      361 tccacttaga cggcgaggac gtggcgatgg cgcatgccga cgcgctagac gatttcgatc
      421 tggacatgtt gggggacggg gattccccgg gtccgggatt taccccccac gactccgccc
      481 cctacggcgc tctggatatg gccgacttcg agtttgagca gatgtttacc gatgcccttg
      541 gaattgacga gtacggtggg gatccgtctg ctggagacat gagagctgcc aacctttggc
      601 caagcccgct catgatcaaa cgctctaaga agaacagcct ggccttgtcc ctgacggccg
      661 accagatggt cagtgccttg ttggatgctg agccccccat actctattcc gagtatgatc
      721 ctaccagacc cttcagtgaa gcttcgatga tgggcttact gaccaacctg gcagacaggg
      781 agctggttca catgatcaac tgggcgaaga gggtgccagg ctttgtggat ttgaccctcc
      841 atgatcaggt ccaccttcta gaatgtgcct ggctagagat cctgatgatt ggtctcgtct
      901 ggcgctccat ggagcaccca gtgaagctac tgtttgctcc taacttgctc ttggacagga
      961 accagggaaa atgtgtagag ggcatggtgg agatcttcga catgctgctg gctacatcat
     1021 ctcggttccg catgatgaat ctgcagggag aggagtttgt gtgcctcaaa tctattattt
     1081 tgcttaattc tggagtgtac acatttctgt ccagcaccct gaagtctctg gaagagaagg
     1141 accatatcca ccgagtcctg gacaagatca cagacacttt gatccacctg atggccaagg
     1201 caggcctgac cctgcagcag cagcaccagc ggctggccca gctcctcctc atcctctccc
     1261 acatcaggca catgagtaac aaaggcatgg agcatctgta cagcatgaag tgcaagaacg
     1321 tggtgcccct ctatgacctg ctgctggaga tgctggacgc ccaccgccta catgcgccca
     1381 ctagccgtgg aggggcatcc gtggaggaga cggaccaaag ccacttggcc actgcgggct
     1441 ctacttcatc gcattccttg caaaagtatt acatcacggg ggaggcagag ggtttccctg
     1501 ccacagtctg agagctccct ggcgaattcc cagagatgtt agctgaaatc atcactaatc
     1561 agataccaaa atattcaaat ggaaatatca aaaagcttct gtttcatcaa aaatgactcg
     1621 acctaactga gtaagctagc ttgttcgagt attatggcat tgggaaaact gtttttcttg
     1681 taccatttgt tgtgcttgta atttactgtg ttttttattc ggttttcgct atcgaactgt
     1741 gaaatggaaa tggatggaga agagttaatg aatgatatgg tccttttgtt cattctcaaa
     1801 ttaatattat ttgttttttc tcttatttgt tgtgtgttga atttgaaatt ataagagata
     1861 tgcaaacatt ttgttttgag taaaaatgtg tcaaatcgtg gcctctaatg accgaagtta
     1921 atatgaggag taaaacatcc caaacgcggc cgcatcgttc aaacatttgg caataaagtt
     1981 tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt
     2041 acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta
     2101 tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa
     2161 actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggga attgatcccc
     2221 cctcgacagc ttgcatgcca gcttgggctg caggtcgagg ctaaaaaact aatcgcatta
     2281 tcatcccctc gacgtactgt acatataacc actggtttta tatacagcag tactgtacat
     2341 ataaccactg gttttatata cagcagtcga cgtactgtac atataaccac tggttttata
     2401 tacagcagta ctgtacatat aaccactggt tttatataca gcagtcgagg taagattaga
     2461 tatggatatg tatatggata tgtatatggt ggtaatgcca tgtaatatgc tcgactctag
     2521 gatcttcgca agacccttcc tctatataag gaagttcatt tcatttggag aggacacgct
     2581 gaagctagtc gactctagca caagtttgta caaaaaagtt gaacgagaaa cgtaaaatga
     2641 tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa
     2701 aacacaacat atgcagtcac tatgaatcaa ctacttagat ggtattagtg acctgtagaa
     2761 ttcgagctct agagctgcag ggcggccgcg atatccccta tagtgagtcg tattacatgg
     2821 tcatagctgt ttcctgaaat cactagtgcg gccgcctgca ggtcgaccat atgggagagc
     2881 tcccaacgcg ttggatgcat agcttgagta ttctatagtg tcacctaaat agcttggcgt
     2941 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     3001 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     3061 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     3121 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     3181 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     3241 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     3301 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     3361 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     3421 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     3481 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     3541 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     3601 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     3661 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     3721 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     3781 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     3841 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     3901 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     3961 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     4021 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     4081 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     4141 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     4201 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     4261 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     4321 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     4381 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     4441 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     4501 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     4561 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     4621 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     4681 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     4741 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     4801 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     4861 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     4921 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     4981 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     5041 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     5101 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gaaattgtaa
     5161 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     5221 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     5281 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     5341 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     5401 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     5461 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     5521 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     5581 cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc aggctgcgca actgttggga
     5641 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     5701 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     5761 cagtgaattg taatacgact cactataggg cgaattgggc ccgacgtcgc atgctcccgg
     5821 ccgccatggc cgcgggatag taaaacgacg gccagtctta agctcgggcc cgcgttaacg
     5881 ctaccatgga gctccaaata atgattttat tttgactgat agtgacctgt tcgttgcaac
     5941 aaattgataa gcaatgcttt tttataatgc caactttgta tag 
//
