After Ghedotti, M. J. 2000, with species from Froese & Pauly, 2001 [“The FishBase”]

<==o Gambusini (mosquitofishes and relatives; moskiittokalat) [FB: 3/47]
   |-- Belonesox belizanus Kner, 1860 (Topminnow)
   |--o Brachyrhaphis [Brachyraphis] ()? [8]
   |  |-- B. cascajalensis (Meek & Hildebrand, 1913)
   |  |-- B. episcopi (Steindachner, 1878)
   |  |-- B. hartwegi Rosen & Bailey, 1963
   |  |-- B. holdridgei Bussing, 1967
   |  |-- B. parismina (Meek, 1912)
   |  |-- B. punctifer (Hubbs, 1926)
   |  |-- B. rhabdophora (Regan, 1908)
   |  |-- B. roseni Bussing, 1988
   |  `-- B. terrabensis (Regan, 1907)
   `--o Gambusia (moskiittokalat; mosquitofishes, gambusias) [Gh: 45] [FB: 38]
      |-- G. affinis (Baird & Girard, 1853) (moskiittokala; Mosquitofish)
      |-- G. alvarezi Hubbs & Springer, 1957
      |-- G. amistadensis Peden, 1973 (Amistad gambusia)
      |-- G. atrora Rosen & Bailey, 1963
      |-- G. aurata Miller & Minckley, 1970
      |-- G. beebei Myers, 1935 (Miragoane gambusia)
      |-- G. clarkhubbsi Garrett & Edwards, 2003 (San Filipe Gambusia)
      |-- G. dominicensis Regan, 1913 (Dominican gambusia)
      |-- G. echeagarayi (Alvarez, 1952)
      |-- G. eurystoma Miller, 1975
      |-- G. gaigei Hubbs, 1929 (Big Bend gambusia)
      |-- G. geiseri Hubbs & Hubbs, 1957 (Largespring gambusia)
      |-- G. georgei Hubbs & Peden, 1969 (San Marcos gambusia)
      |-- G. heterochir Hubbs, 1957 (Clear Creek gambusia)
      |-- G. hispaniolae Fink, 1971 (Hispaniolan gambusia)
      |-- G. holbrooki Girard, 1859 (Eastern mosquitofish)
      |-- G. hurtadoi Hubbs & Springer, 1957
      |-- G. krumholzi Minckley, 1963
      |-- G. lemaitrei Fowler, 1950
      |-- G. longispinis Minckley, 1962 (Cuatrocienegas gambusia)
      |-- G. luma Rosen & Bailey, 1963 (Sleek mosquitofish)
      |-- G. marshi Minckley & Craddock, 1962
      |-- G. melapleura (Gosse, 1851) (Striped gambusia)
      |-- G. myersi Ahl, 1925
      |-- G. nicaraguensis Günther, 1866 (Nicaraguan mosquitofish)
      |-- G. nobilis (Baird & Girard, 1853) (Pecos gambusia)
      |-- G. panuco Hubbs, 1926
      |-- G. pseudopunctata Rivas, 1969 (Tiburon Peninsula gambusia)
      |-- G. punctata Poey, 1854 (Cuban gambusia)
      |--o G. puncticulata Poey, 1854 (Caribbean gambusia)
      |  |-- G. p. baracoana Rivas, 1944
      |  |-- G. p. bucheri Rivas, 1944
      |  |-- G. p. manni Hubbs, 1927
      |  |-- G. p. monticola Rivas, 1971
      |  |-- G. p. puncticulata Poey, 1854 (Caribbean gambusia)
      |  `-- G. p. yucatana Regan, 1914
      |-- G. rachowi (Regan, 1914)
      |-- G. regani Hubbs, 1926
      |-- G. rhizophorae Rivas, 1969 (Mangrove gambusia)
      |-- G. senilis Girard, 1859 (Blotched gambusia)
      |-- G. sexradiata Hubbs, 1936 (Teardrop mosquito)
      |-- G. speciosa Girard, 1859
      |-- G. vittata Hubbs, 1926
      |-- G. wrayi Regan, 1913 (Wray's gambusia)
      `-- G. xanthosoma Greenfield, 1983 (Cayman gambusia)

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