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REMEDIC is supported by

the European Science Foundation
 


 

Introduction

The Department of Neuroscience consists of two laboratories: the Laboratory of Tissue Culture and Stem Cells and the Laboratory of Diffusion Studies and Imaging Methods.

Research in the Laboratory of Tissue Culture and Stem Cells focuses on these main topics: the characterization of adult and embryonic stem cells in vitro, the development of nanoparticles for cell labeling suitable for in vivo cell tracking, the regeneration and repair of stroke lesions using stem cells, the regeneration and repair of injured spinal cord using stem cells and biomaterials such as nanofibers and polymer hydrogels, the use of cell-polymer constructs to bridge lesions of the central nervous tissue, the use of nanofiber scaffolds for two- and three-dimensional cell cultivation and as cell carriers in transplantation therapies, the cultivation and differentiation of human embryonic stem cells into a neuronal phenotype, the phenotyping of stem cells by means of flow cytometry, and the translation of pre-clinical findings into human clinical practice, including conducting phase I/II clinical trials in conjunction with Motol Faculty Hospital in Prague.

Research in the Laboratory of Diffusion Studies and Imaging Methods focuses on these main topics: the origin, mechanisms and pathophysiological significance of ionic changes in the extracellular space, diffusion in the extracellular space as the underlying mechanism of extrasynaptic ("volume") transmission, diffusion studies using the real-time TMA+ iontophoretic method as well as diffusion-weighted MR, extracellular space volume and geometry as factors affecting diffusion in the CNS in health and disease, and studies using models of pathological states, including transgenic animals.

 

Director:

 

Professor Eva Syková, MD, DSc
Department of Neuroscience
Institute of Experimental Medicine ASCR, v.v.i.                   
Vídeňská 1083
14220 Prague 4, Czech Republic
Phone: +420 241 062 230
Fax: +420 241 062 782

 

Eva Syková

Professor Eva Syková, M.D., Ph.D, D.Sc., is the head of the Department of Neuroscience and director of the Institute of Experimental Medicine ASCR, an EU Centre of Excellence, and is also the head of the Center for Cell Therapy and Tissue Repair at Charles University in Prague. She has published 175 papers in peer-reviewed journals, 35 book chapters, is the author of 1 book and the co-editor of 4 books. Her publications have been cited more than 5500 times; her Hirsch index is 41. She is a co-holder of 5 patents. She has given more than 100 invited lectures and seminars at foreign universities and international conferences and has co-organized 14 international meetings and 4 teaching workshops. Among her scientific awards are the prizes of the Czech Physiological Society, the Czech Neuroscience Society, the Czech Medical Society, the Czech Academy of Sciences, and the Purkyně medal for her merit in biological sciences. She has been elected a member of Academia Europea, the Czech Medical Academy and the Russian Academy of Natural Sciences. She serves on the editorial boards of 6 international journals and as a member of the executive committees of a number of Czech and international scientific societies and boards. Since 2005 she has been the President of the Czech Neuroscience Society.

 

Group members


The staff of the Department of Neuroscience comprises approximately 25 individuals, including technicians, PhD students, post-docs and senior scientists from the Czech Republic and five other countries. They are joined by a number of foreign visitors every year.

The staff of the Laboratory of Tissue Culture and Stem Cells

 

The staff of the Laboratory of Diffusion Studies

Publications
Some representative recent publications:

Jendelová P, Herynek V, Urdzíková L, Glogarová K, Kroupová J, Bryja V, Andersson B, Burian M, Hájek M, Syková E: MR tracking of transplanted bone marrow and embryonal stem cells labeled by iron oxide nanoparticles in rat brain and spinal cord. J Neurosci Res 76: 232-243, 2004.
Urdzikova L, Jendelova P, Glogarova K, Burian M, Hajek M, Sykova E: Transplantation of bone marrow stem cells as well as mobilization by granulocyte-colony stimulating factor promotes recovery after spinal cord injury in rats. J Neurotrauma 23:1379-91, 2006.
Sykova E, Homola A, Mazanec R, Lachmann H, Konradova SL, Kobylka P, Padr P, Neuwirth J, Komrska V, Vavra V, Stulik J, Bojar M: Autologous bone marrow transplantation in patients with subacute and chronic spinal cord injury. Cell Transplantation 15: 675-687, 2006.
Syková E, Jendelová P, Urdzíková L, Lesny P, Hejčl A: Bone marrow stem cells and polymer hydrogels - two strategies for spinal cord injury repair. Cell Mol Neurobiol 26: 1113-29, 2006.
Syková E, Jendelová P: Migration, fate and in vivo imaging of adult stem cells in the CNS. Cell Death Differ 14: 1336-42, 2007.
Hejcl A, Urdzikova L, Sedy J, Lesny P, Pradny M, Michalek J, Burian M, Hajek M, Zamecnik J, Jendelova P, Sykova E: Acute and delayed implantation of positively charged 2-hydroxyethyl methacrylate scaffolds in spinal cord injury in the rat. J Neurosurg Spine 8: 67-73, 2008.
Syková E, Nicholson C: Diffusion in brain extracellular space. Physiol Rev 88: 1277-1340, 2008.
Kozubenko N, Turnovcova K, Kapcalova M, Butenko O, Anderova M, Rusnakova V, Kubista M, Hampl A, Jendelova P, Sykova E: Analysis of in vitro and in vivo characteristics of human embryonic stem cell-derived neural precursors. Cell Transplant 19: 471-486, 2010.
Hejcl A, Sedý J, Kapcalová M, Arboleda Toro D, Amemori T, Likavčanová-Mašínová K, Lesný P, Krumbholcová E, Přádný M, Michálek J, Burian M, Hájek M, Jendelová P, Sykova E. HPMA-RGD hydrogels seeded with mesenchymal stem cells improve functional outcome in chronic spinal cord injury. Stem Cells Dev 2010 Jan 6.
Kubinová S, Syková E: Nanotechnology for treatment of stroke and spinal cord injury. Nanomedicine (Lond.) 5: 99-108, 2010.