Institute of Experimental Medicine
Academy of Sciences of the Czech Republic, v.v.i.
Vídeňská 1083
14220 Prague 4, Czech Republic

A number of experimental methodologies and equipment are currently in use in the Department of Neuroscience. Among the methodologies used on a regular and ongoing basis are magnetic resonance imaging (MRI), magnetic resonance spectroscopy (MRS), functional magnetic resonance imaging (fMRI), diffusion-weighted magnetic resonance imaging (DW-MRI), culturing of stem and progenitor cells, behavioral techiques for testing motor, sensory and memory deficits, the TMA+ real-time iontophoretic method of diffusion analysis, the measurement of extracellular ionic concentrations using ion-selective microelectrodes, intrinsic optical imaging, the patch-clamp technique, multichannel recording, immunohistochemistry, image analysis techniques, single and double photon confocal microscopy, electron microscopy and fluorescein-activated cell sorting (FACS).

To enable the use of these methods, the available equipment includes an animal house and breeding facility, a tissue culture laboratory, a histology and immunohistochemistry laboratory, a small animal surgical theater, a Leica confocal microscope, a Leica image analysis system, a Morgagni transmission electron microscope, several Zeiss fluorescent microscopes, a Zeiss image analysis system, a Bruker 4.7 Tesla NMR specially designed for small animal studies, a 1.5 Tesla Siemens Vision fNMR and a Becton Dickinson FACSAria flow cytometer.

Patch-Clamp Set-up

Leica confocal microscope

Morgagni transmission electron microscope