Extracellular Vesicle - Nanoparticle Tracking Analysis (NTA)

 

Extracellular Vesicle | Apogee A50 Micro Flow Cytometer | Nanoparticle Tracking Analysis (NTA) | Services and Fees | Reservations and contact


Nanoparticle tracking analysis (NTA) is a method used to quantify and determine the size distribution of nanoparticles ranging from 30 nm to 1 µm of diameter. The sample is injected to the measuring chamber and the sample particles are visualized using a laser beam. Particles pass through the beam path are seen as small points of light moving rapidly under Brownian motion, allowing information on particle properties to be obtained.

The Nanopartice Tracking Analysis provides characterization of

  • extracellular vesicles including exosomes
  • microparticles
  • nanoparticles

Instrument

Nanosight LM14C

  • Blue laser 405 nm, 60 mW
  • CMOS camera
  • Nanosight software v3.0
  • Minimum volume 500 µl
  • Optimal concentration of sample is around 5 x 108 particles/ml
  • Results are provided in PDF and excel files

Service

EV-core Viikki provides NTA measurement and data analysis.

Minimum charge is for 1 h, after which every starting ½ hour is charged. The minimum time includes the baseline buffer run. Running one sample takes typically 5-10 minutes and the analysis of the sample 15-20 minutes, during which the equipment is washed.

Basic solutions and disposables are provided. The used sample buffer should be filtrated with 0,1 µm filtration unit to prevent any external particles in the sample.