Extracellular Vesicle - Apogee A50 Micro Flow Cytometer

 

Extracellular Vesicle | Apogee A50 Micro Flow Cytometer | Nanoparticle Tracking Analysis (NTA) | Services and Fees | Reservations and contact


The Apogee A50- Micro flow cytometry is specifically designed for measuring sub-micron biological particles using different fluorescently labelled probes. With apogee A50 it is possible to analyze particles >100 nm with resolving power <10 nm. The use of Apogee A50 requires some prior concentration estimation of the sample e.g. with nanoparticle tracking analysis (NTA) available in our lab.

The Apogee A50- Micro provides accurate analysis and fluorescence measurements of:

  • extracellular vesicles including exosomes
  • microparticles
  • nanoparticles
  • platelets

Instrument

  • Two lasers: violet 405 nm and blue 288 nm.
  • Three fluorescent detectors for green, orange and red together with two scatter parameters.
  • Minimum sample volume 150 µl, max sample volume 400 µl.
  • Working range from >100- 25 000 events/s, recommended range 500-10 000 events/s
  • Data from Apogee Histogram can be exported to excel format

Service

EV-core Viikki provides micro flow cytometry analysis services.

Minimum charge is 1 h, after which every starting ½ hour is charged. The minimum time includes two sets of control beads and the baseline buffer run. Running one sample takes typically 2-5 minutes followed by 1 minute flushing cycle. The washing steps after samples are stringent and take from 20 minutes to 1 hour, depending on the number and concentration of samples. 

Basic solutions and disposables are provided. In case the user is not providing fluorescently labelled probes please contact the unit manager for possibility to use EV-unit’s probes for additional fee.