Division of Biochemistry and Biotechnology - FACS Unit

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BD LSR II FLOW CYTOMETRY

Flow cytometer can be used to measure different parameters, e.g. intensity of fluorescence
at different wavelengths and/or forward and side scatter of light, of a living cell in suspension. Cell surface antigens or intracellular molecules can be labeled with an antibody coupled to a fluorochrome and the intensity of the fluorescence gives information of the expression of the molecule in question. In addition, forward and side scatter tell about the size and granularity
of the cell.

Division of Biochemistry has a new BD LSR II flow cytometer that was bought as a collective purchase in September 2005. The instrument is able to measure 6 different fluorescence parameters in addition to forward and side scatter. It is controlled by FACSDiva software.

The device is situated in Viikki, Biocenter 2, Division of Biochemistry, Viikinkaari 5 D, 3 rd floor, room 3017. The fees will be negotiated according to the nature of the use for others
than those participating in the collective purchase.

We have also a BD FACScan flow cytometer that can be used if needed.

Lasers, excitation wavelengths, fluorescence channels and examples of possible fluorochromes:

Blue 488 nm argon-ion laser

Green FITC, Alexa Fluor 488, EGFP
Yellow PE, EYFP
Red PerCP, PerCP-Cy5.5, Pe-Cy5, PE-Texas Red, ds Red
Infra Red Pe-Cy7

Red 635 nm He-Ne laser

Red APC, Alexa Fluor 647
Infra Red APC-Cy7

Customer Prices:

Basic charge 25 € (Paid every instance of use)
Hourly charge 20 € / hour
Customer training (LSRII) 40€

VAT (23 %) not included

Contact information:

Maria Semenova, M.Sc
Department of Biosciences
Division of Biochemistry
P.O. Box 56
Viikinkaari 5 D, 3rd floor, room 3017
00014 UNIVERSITY OF HELSINKI

Tel: 191 59035, 050-4154463
Fax: 09-19159068
Email: maria.m.semenova(at)helsinki.fi

Links:

>> BD Biosciences online course on flow cytometry

>> BD LSR II, supplementary information of the instrument