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Microinjection and Imaging unit


Microinjection and Imaging Unit of the Department of Biological and Environmental Sciences in Biocenter 2 serves research groups and individual researchers of the Viikki Biocenter and also groups in other institutions in Helsinki. Service is also provided for commercial users. In microinjection techniques we give full service, assisted use of the equipment and independent work in the unit. Our staff provides training and consultation concerning the techniques. The unit organizes practical courses at under graduate and graduate level.

The instruments of the unit consist of two sets of microinjection instruments. One is Eppendorf semiautomatic injection unit connected to Zeiss Axiovert 100 inverted microscope and the other one is a Narishige manual injector connected to a Leica DM IRB microscope. Both of the instruments are equipped with camera and computer. Cell culture facilities such as microscope, incubator and laminar are available in the injection room. Microinjection and Imaging Unit is funded by the Department of Biological and Environmental Sciences and Biocentrum Helsinki.

Instruments:

Eppendorf semiautomatic microinjector is equipped with:

-Inverted Zeiss Axiovert microscope
-COHU High Performance CCD camera, Sony monitor
-Photometrics SenSys 1400 CCD camera
-a computer with Media Cybernetics Image Pro Plus imaging software
-Panasonic Play Shop time lapse video recorder

Objectives:

Apoplan 4x/0,1 (air)
Plan NeoFluor ph1 10x/0,3(air)
Achroplan 20x/0,4 (air)
Achroplan 40x/0,6 (air)
Neofluor 100x/1,3 (oil)

Contact information:

Esa Kuismanen, PhD, Docent
Responsible for Eppendorf injector, consultation and introduction to microinjection techniques
tel.09-191 590 55
e-mail: esa dot kuismanen at helsinki dot fi


Anne Makkonen
, technician
Responsible for Eppendorf injector reservations
tel: .09-191 590 57
e-mail: anne dot m dot makkonen at helsinki dot fi